Figure 3.

H₂O₂-Induced Programmed Cell Death and Increased Cell Death in PLDδ-Null Leaves.

(A) TUNEL staining of H₂O₂-induced programmed cell death in wild-type (WT) and PLDδ-null protoplasts. 3′-OH groups of DNA were visualized as bright green spots within the nucleus. Protoplasts were incubated with 1 mM H₂O₂ for 3 h before DNA was labeled using the TUNEL method.

(B) Trypan blue staining of cell death in wild-type and PLDδ-null Arabidopsis leaves. Leaves of 5-week-old plants were infiltrated with 50 mM H₂O₂ or water. The arrow indicates dead cells.