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Ablation of H2O2-Promoted MAPK Activity in PLDδ-Null Arabidopsis.
Autoradiogram from an in-gel kinase assay using protein extracts from wild-type (WT) and PLDδ-null Arabidopsis protoplasts. Myelin basic proteins were embedded on a 10% polyacrylamide gel as the substrate. After electrophoresis, proteins were renatured on the gel and assayed for phosphorylation activity using γ-32P-ATP.
