Figure 4.

Intracellular Localization of the Pchlide That Accumulates in the flu Mutant.

(A) Tetrapyrroles were extracted from etiolated seedlings of wild-type (wt) and flu plants, separated by HPLC, and detected by their fluorescence.

(B) Colocalization of the PORA-GFP fusion protein (a) and free Pchlide (c) in transgenic flu plants. Pchlide was not detected in the cytoplasm outside of the plastids, as indicated by the distribution of the GFP without the PORA signal sequence attached to it ([b] and [d]). Bars = 5 μm.