Figure 3.

Defense Gene Expression in the sfd Mutants.

(A) Comparison of PR1 and BGL2 expression in leaves of 4-week-old soil-grown wild-type (WT), npr1, ssi2, ssi2 npr1, sfd1-1 ssi2 npr1, sfd1-2 ssi2 npr1, sfd4 ssi2 npr1, sfd2-3 ssi2 npr1, sfd2-2 ssi2 npr1, sfd2-1 ssi2 npr1, and sfd2-1 ssi2 plants.

(B) Comparison of PR1 expression in leaves of 4-week-old wild-type, npr1, ssi2, ssi2 npr1, sfd1-1 ssi2 npr1, and sfd2-1 ssi2 npr1 plants 48 h after treatment with 500 μM SA.

(C) Comparison of PR1 expression in leaves of 4-week-old soil-grown ssi2, ssi2 npr1, wild-type, npr1, and sfd1-1 ssi2 plants.

(D) Comparison of PR1 expression in sfd2-1 ssi2 npr1, sfd2-1/+ ssi2 npr1, and ssi2 npr1 plants.

(E) Comparison of PDF1.2 expression in leaves of 4-week-old wild-type, npr1, ssi2, ssi2 npr1, ssi2 npr1 nahG, sfd1-1 ssi2 npr1, sfd4 ssi2 npr1, and sfd2-1 ssi2 npr1 plants 48 h after treatment with 5 μM MeJA dissolved in 0.1% ethanol.

All RNAs were resolved on denaturing gels, transferred to Nytran Plus membranes (Schleicher & Schuell), and probed for the indicated genes. Gel loading was monitored by photographing the ethidium bromide–stained gel (EtBr) before transferring the RNA to a Nytran Plus membrane.