Figure 5.

Promoter Constructs Used in Transient Gene Expression Analysis and Ubi1 Expression in Developing Aleurone Layers.

(A) Schemes of constructs used in the experiments testing the effectiveness of antisense MuDR fragments in reducing corresponding sense transcript. PCR-amplified MuDR fragments were cloned into the 3′ region of pAHC17 to make a transcriptional fusion with the DNA segment in an antisense orientation with respect to the ubiquitin promoter. Numbers on each PCR fragment represent the positions in MuDR. Drawings are not to scale. CaMV, Cauliflower mosaic virus; Nos, nopaline synthase.

(B) Ubi1 expression in the aleurone layers of developing kernels. Total RNAs were prepared from aleurone layers of developing kernels at different days after pollination (D.A.P.). The RNAs were fractionated on a 1.2% agarose gel and probed with a Ubi1-specific oligonucleotide.