Table 7.
Effect of Antisense mudrA Expression on the Frequency of Mu1 Somatic Excision from bz2-mu2
| Excision Frequency
|
|||
|---|---|---|---|
| Sample | High (>150 a) | Medium (10 to 150) | Low (<10) |
| Anti-mudrA (−) | 65.0 ± 14.0 b | 30.2 ± 11.4 | 4.0 ± 3.2 |
| Anti-mudrA (+) | 66.4 ± 9.4 | 27.8 ± 8.1 | 5.8 ± 2.8 |
T3 progeny ears described in Table 6 were sampled from transgenic plants carrying Anti-mudrA-4 or Anti-mudrA-5.
The number of excision spots in a 2 mm × 2 mm area was counted for each kernel using a Nikon stereo zoom microscope. Most excision sectors consist of 1 to 16 cells, and there was no significant difference in the excision timing depending on the expression of the transgene (data not shown).
Spotted kernels were classified by their excision frequency as high, medium, and low. Numbers represent the percentage of kernels on each ear that belong to the indicated frequency category ± sd. Data were taken from counting of 1235 spotted kernels/8 ear families that do not express the transgene and of 1085 spotted kernels/7 ears families that express the antisense mudrA transgene.