Table 1.
Reactions from the E. coli central metabolic network
| Reaction | Gene name | Enzyme | Chemical reaction |
| 1 |
ptsH, ptsI crr, glk |
Glucose phosphotransferase system | Glc + PEP => G6P + PYR |
| 2 |
pfkA, pfkB fba tpi |
Phosphofructokinase Fructose-16-bisphosphatate aldolase Triphosphate isomerase |
F6P => 2 GAP |
| 3 |
gapA pgk gpmA |
Glycelaldehyde-3-phosphate dehydrogenase Phosphoglycerate kinase Phosphoglycerate mutase I |
GAP => PEP |
| 4 |
pykF pykA |
Pyruvate kinase I | PEP => PYR |
| 5 |
aceE aceF lpdA |
Pyruvate dehydrogenase | PYR => AcCoA |
| 6 | pta, ackA | Acetyl-CoA synthetase | AcCoA => Acetate |
| 7 |
gltA acnA, acnB |
Citrate synthase Aconitase A |
AcCoA + OAA => ICT |
| 8 | icdA | Isocitrate dehydrogenase | ICT => AKG |
| 9 |
sucA, sucB, lpdA sucC, sucD sdhC fumA, fumB, fumC |
2-ketoglutarate dehydrogenase Succinyl-CoA synthetase Succinate dehydrogenase Fumarase |
AKG => MAL |
| 10 | mdh | Malate dehydrogenase | MAL => OAA |
| 11 | ppc | Phosphenolpyruvate carboxylase | PEP => OAA |
| 12 | mez | Malic enzyme | MAL => PYR |
| 13 | zwf | Glucose-6-phosphate-1-dehydrogenase | G6P => 6PG |
| 14 | gnd | 6-phosphoglycononate dehydrogenase | 6PG => Ru5P |
| rpiA | Ribose-5-phosphate isomerase A | Ru5P => R5P | |
| rpe | Ribose phosphate 3-epimerase | Ru5P => X5P | |
| 15 | tktA | Transketolase I | X5P + R5P => GAP + Sed7P |
| 16 | tktB | Transketolase II | X5P + E4P => F6P +GAP |
| 17 | talB | Transaldolase B | GAP + Sed7P => F6P + E4P |
| 18 | pgi | Phosphoglucoisomerase | G6P => F6P |
| 19 | Glucose uptake | Glc_ext => Glc | |
| 20 | Materials are used for biomass synthesis | G6P => Biomass | |
| 21 | Materials are used for biomass synthesis | F6P => Biomass | |
| 22 | Materials are used for biomass synthesis | GAP => Biomass | |
| 23 | Materials are used for biomass synthesis | PEP =-> Biomass | |
| 24 | Materials are used for biomass synthesis | PYR => Biomass | |
| 25 | Materials are used for biomass synthesis | AcCoA => Biomass | |
| 26 | Membrane transport reaction | Acetate => Acetate_ext | |
| 27 | Materials are used for biomass synthesis | AKG => Biomass | |
| 28 | Materials are used for biomass synthesis | OAA => Biomass | |
| 29 | Materials are used for biomass synthesis | R5P => Biomass | |
| 30 | Materials are used for biomass synthesis | E4P => Biomass |
Abbreviation: AcCoA Acetyl-CoA, AKG α-ketoglutarate, E4P erythrose-4-phosphate, F6P fructose-1,6-bisphosphate, Glc glucose, GAP glyceraldehydes-3-phosphate, G6P glucose-6-phosphate, ICT isocitrate, Mal malate, OAA oxaloacetate, PEP phosphoenolpyruvate, 6PG 6-phosphogluconate, Ru5P ribulose-5-phosphate, X5P, xylulose 5-phosphate, R5P ribose-5-phosphate, Sed7P sedoheptulose-7-phosphate, Glc_ext Glucose external, Acetate_ext Acetate external.