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. Author manuscript; available in PMC: 2007 Sep 7.
Published in final edited form as: Mol Genet Metab. 2007 Mar 21;91(1):85–97. doi: 10.1016/j.ymgme.2007.02.001

Fig. 3.

Fig. 3

Western blotting of 100,000 × g supernatants of liver (L), heart (H), kidney (K) and brain (B) tissue homogenates from Mtrr+/+ and Mtrrgt/gt female mice was performed to assess the impact of the gene trap insertion on protein levels. The response to polyclonal antibody specific for MTRR protein was assessed (top panel). Mouse MTRR migrates at the correct molecular mass of 77.6 kDa. The purified recombinant human methionine synthase reductase with N-terminal hexahistidine tag (MTRR 6xHis) migrates at 81.1 kDa. The blot was stripped and re-probed with antibody specific for the 42.1 kDa mouse β-actin protein (lower panel), in order to confirm equal protein loading, as shown in the bottom panel.