Figure 1.

Identification and Isolation of EPR1.

(A) FDD screening for CB18-490. Total RNA was harvested from etiolated wild-type seedlings at 0, 30, and 60 min of dark incubation after 1 mmol m⁻² R light irradiation.

(B) RNA gel blot analysis confirming the observed R light–induced expression of the isolated gene corresponding to the CB18-490 FDD fragment in (A). Total RNA (20 μg per lane) from etiolated wild-type seedlings at 0, 30, 60, 120, and 240 min of dark incubation after 1 mmol m⁻² R light irradiation was hybridized with the CB18-490 probe. The transcript sizes were estimated using RNA size markers (0.16- to 1.77-kb RNA ladder). 18S rRNA was used for normalization.

(C) Nucleotide and deduced amino acid sequences of EPR1. Boldface letters indicate the arbitrary (OPB-18) and oligo(dT) primers that were used for FDD reactions. The amino acid sequence of the EPR1 MYB domain is underlined.

(D) Comparison of the MYB domain amino acid sequences of EPR1, LHY, and CCA1. Identical amino acid residues are boxed, asterisks represent the positions of conserved Trp and Ala residues in the MYB domain, and dashes indicate gaps.