FR Light–Induced Cotyledon Opening of Transgenic Arabidopsis Overexpressing EPR1.
(A) RNA gel blot analysis (20 μg of total RNA) of wild-type Columbia (Col) and four transgenic lines overexpressing EPR1.
(B) Cotyledon opening of wild-type Columbia and EPR1-overexpressing line 19 (EPR1 ox-19) was induced by intermittent FR light irradiation.
(C) Cotyledon angles of the wild type (WT) and EPR1-overexpressing lines 19 and 25 (EPR1 ox-19 and EPR1 ox-25) at different fluences of FR light pulses. Accompanying statistical analysis (t test) showed that the differences in cotyledon angles were significant. D, darkness.