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Synthetic transcriptional activators in yeast. (A) Schematic diagram of an activator cascade composed of an activator gene and a reporter gene. (B) DIC and fluorescence images of live cells containing the ZifH activator cascade in the absence (−galactose) and presence (+galactose) of the inducer or only the ZifH reporter gene in the presence of the inducer. (C) Dot plots of YFP versus RFP fluorescence intensity for single cells grown as in B.
