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. Author manuscript; available in PMC: 2008 May 10.
Published in final edited form as: Vaccine. 2007 Feb 15;25(19):3841–3850. doi: 10.1016/j.vaccine.2007.01.107

Fig. 5.

Fig. 5

HIV Env peptide specific cytokines produced by spleen cells. A) IFN-γ ELISA. B) IL-6 ELISA. The culture supernatants of splenocytes after stimulation with an HIV Env 89.6 peptide were used to determine cytokines IFN-γ and IL-6 using ELISA. C to E) Cytokines (IL-2, 4, 5) determined by ELISPOT. The spots for cytokine-producing splenocytes after stimulation with an HIV Env 89.6 peptide were counted and expressed based on 1.5 × 106 cells. Bars indicate standard deviations. Immunization groups are the same as described in Fig. 4. Statistically significant differences between WT and mutants are indicated as * (P <0.05).