Table 1.
Nucleotide sequences of primers and RT-PCR conditions for analysis of ANTXR mRNA expression
| GeneBank Access | Expected Product Size | Primer sequences | RT-PCR reaction conditions | |
|---|---|---|---|---|
| ANTXR1 | NM054041 | 231 bp (975–1205) | F: 5′-CTTTCAAGTGGTCGTAAGAG-3′
R: 5′-GTGATGATGACAAGAACTGGA-3′ |
RT: 50°C 30 min.
94°C 15 min. 1 cycle |
| ANTXR2 | NM 33738 | 242 bp (1088–1330) | F: 5′-TCCTCCAAGTGTCTGTGTGTAG-3′
R: 5′-GGCTGTGATTGTTAAGGATC-3′ |
PCR: 94 °C 15 s
55 °C 30 s 72 °C 30 s 40 cycles |
| 18S rRNA | AY248756 | 101 bp (950–1050) | F: 5′-CGCCGCTAGAGGTGAAATTCT-3′
R: 5′-CGAACCTCCGACTTTCGTTCT-3′ |