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. Author manuscript; available in PMC: 2008 Jun 22.
Published in final edited form as: Mol Cell. 2007 Jun 22;26(6):883–890. doi: 10.1016/j.molcel.2007.05.020

Figure 2. Trans-splicing does not occur in reporter genes with mutations outside the 5’SS.

Figure 2

(A). Schematic of ACT1-CUP1 reporter pre-mRNA and U2 snRNA, indicating the mutations used in panels C and D, and the schematic location of RT and PCR primers (arrows) used in panel B.

(B). Mutations at and around the branch site do not stimulate trans-splicing in an otherwise wild-type context, and inhibit it in the context of an accompanying 5’SS mutation. RT-PCR analysis of RNA recovered from Y04999 cells containing the ACT1-CUP1 reporters as indicated, as in Fig. 1C.

(C). Primer extension analysis of RNA recovered from Y04999 cells containing the ACT1-CUP1 reporters as indicated, as in Fig. 1D.