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Diagram of α2M monomer indicating regions important to its function. Aligned below the monomer is the fusion protein encoded by the prey plasmid B42-α2M, containing B42 activation domain followed in frame by the final 250 amino acids of the α2M C-terminus from Ser 1202 to Ala 1451. Prey plasmid sequence does not include the protease binding and cleavage regions of α2M. However, it incorporates the entire receptor binding site of α2M.
