Figure 2.

O-nitrophenyl-β-d-galactoside assay to assess transcriptional activation of the lacZ reporter gene. Yeast strains were incubated in Gal/Raf complete minimal media for 0, 5, and 10 hr. Then cells were lysed, and β-gal activity was determined using the O-nitrophenyl-β-d-galactoside method. Yeast strains contained the following prey/bait plasmids: strain 1, B42-α2M/LexA-Aβ; strain 2, B42-α2M/LexA-bicoid; strain 3, B42 only/LexA-Aβ; and strain 4, B42 only/Lex A only. Numbers are representative of a set of three independent experiments.