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. 1998 Mar 17;95(6):3275–3280. doi: 10.1073/pnas.95.6.3275

Figure 3.

Figure 3

Bait-prey complexes from yeast cell extracts were immunoprecipitated with anti-Aβ mAbs 4G8 and 6E10. Immunoprecipitates were run on a 10–20% SDS/PAGE Tris-N-[tris(hydroxymethyl)methyl]glycine gel. Gels were blotted and then blots developed with an anti-hemagglutinin antibody recognizing the prey fusion protein. A band of ≈40 kDa corresponding to this protein was detected in immunoprecipitated extracts from cells grown in the presence of galactose (lane 1) but not in the presence of Glc (lane 2). The same 40-kDa band was seen in cell extracts not subjected to immunoprecipitation from cells grown in the presence of galactose (lane 3) but not in the presence of Glc (lane 4). No 40-kDa band was seen in immunoprecipitated extracts from a strain not containing prey cDNA (B42 only/LexA-Aβ) grown in Glc medium (lane 5) or in the EGY48 strain, which does not have bait or prey inserts and was grown in rich medium (lane 6). The gel is representative of a set of three independent experiments.