Figure 4.

Binding between synthetic Aβ 1–40 and purified human α2M. A 0.5 μl of a 1 mg/ml solution of Aβ was incubated overnight at room temperature with 10 μl of a 4 mg/ml solution of α2M. Electrophoresis was carried out using 4–15% SDS/PAGE. Immunoblots were developed by using anti-Aβ antibodies 4G8 or 6E10. Lanes: 1, Aβ alone; 2, α2M alone; 3, Aβ incubated with α2M; and 4, Aβ incubated with hydroxylamine-treated α2M. The gel is representative of a set of three independent experiments.