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Binding of end-biotinylated synthetic Aβ 1–40 with TAG-labeled proteins. A range of concentrations of Aβ peptide labeled with N-terminal biotin was incubated with fixed concentrations of TAG-labeled proteins at room temperature for 2 hr with shaking. The reaction mixture was then introduced into a electrochemiluminescent analyser. The intensity of chemiluminescence measured was plotted against the Aβ concentration to determine Kd (see Table 1).
