Figure 6.

Thioflavine T assay for monitoring Aβ aggregation. Aβ 1–40 (25μM) was incubated at 37°C for 4 days in 1× PBS with α2M, ApoJ, and α1ACT, each at a range of concentrations. IC₅₀ for α2M–Aβ interaction was 280 nM (SEM = 20); IC₅₀ for ApoJ–Aβ interaction was 120 nM (SEM = 20). Data for each protein represent sets of at least three independent experiments.