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Expression of the three subunits of ENaC in a stably transfected MDCK cell line. Wild-type (lanes 1–3) and αβγ-MDCK cells (lanes 4–6) were labeled with [35S]methionine, followed by immunoprecipitations with specific anti-α (lanes 1 and 4), anti-β (lanes 2 and 5), and anti-γ (lanes 3 and 6) antibodies. Bar indicates molecular mass of 85 kDa.
