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Phosphopeptide analysis of the β subunit. Immunoprecipitated β subunit was digested with chymotrypsin, resolved in two dimensions on TLC plates, and visualized by autoradiography. (A) Nonstimulated cells. (B) Aldosterone for 3 h. (C) Insulin. (D) Peptides generated by chymotrypsin digestion of the carboxyl terminus of the β subunit.
