Figure 1.

Isolation of syringolide binding protein(s) by affinity chromatography. (A) Specific binding activity of ¹²⁵I-syringolide 1 to soybean leaf soluble proteins recovered from a syringolide affinity column. (B) Detection of syringolide binding proteins using the native gel binding assay as described in Materials and Methods, with specific bound radioactivity shown by the arrows. CR denotes crude extracts. Unbound ¹²⁵I-syringolide 1 occurs at the bottom of the gel.