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125I-syringolide 1 binding activity of soybean protein produced as His-tagged fusions (Fig. 5). (A) Binding activity in the native gel assay. Four micrograms of protein was loaded in each lane. (B) Quantitation of A of the protein–125I-syringolide complexes by scintillation counting. −, absence of competitive cold syringolide 1; +, presence of 1,500-fold cold syringolide 1.
