Figure 5.

DNA histograms of asynchronous cultures of transformed trx1, trx2 mutant cells. Cells were grown in YMGal medium to exponential phase and prepared for flow cytometry. In A the relative fluorescence intensity is plotted against the relative cell number. The two peaks observed for yTRX1, AtTRX1, … , AtTRX5, and NtTRX1 represent 1n and 2n DNA contents. For each sample, between 10,000 and 20,000 fluorescent events were measured. The proportion of cells in G₁ phase estimated by fried software analysis is indicated in B.