Figure 1.

Isolation and molecular characterization of a zebrafish rtf1^kt641 mutant defective in somite formation. (A–F) Lateral views of wild-type (wt) (A,B,E) and kt641 homozygotes (C,D,F) at the 17-somite stage (A–D) and at 36 h post-fertilization (E,F). Panels (B) and (D) show views of panels (A) and (C) respectively, at higher magnification. Somite boundaries are disrupted in the posterior trunk of the kt641 mutant (square bracket in (D)). At later stages, the kt641 mutation causes reduced pigmentation, limited tail growth and abnormal heart (arrow) and ear (arrowhead) development (F). (G) Meiotic and physical mapping of the kt641 mutation. Horizontal grey bars represent contiguous sequences deposited in linkage group 13. (H) Schematic diagrams of zebrafish Rtf1 proteins encoded by wild-type and kt641 alleles.