Figure 2.

The rtf1^kt641 mutation reduces the expression of genes involved in somite segmentation. (A–E) A comparative representation of expression patterns of her1 (A–C), her7 (D) and deltaC (E) messenger RNAs between wild-type siblings (wt; left halves) and rtf1^kt641 mutants (kt641; right halves) indicates a reduction of these genes in rtf1^kt641 mutants. Each half panel represents the cyclic expression patterns of wild-type siblings and rtf1^kt641 mutants at comparative oscillation phases. Three consecutive expression phases of her1 mRNA are represented (A–C). Arrowheads indicate the anterior-most stripes in rtf1^kt641 mutants, which are shifted in the posterior direction. (F–I) Expression patterns of fgf8 (F,G) and her13.2 (H,I) mRNA in wild-type siblings (F,H) and rtf1^kt641 mutants (G,I). (J,K) her1 nascent transcript hybridized with intron probe in wild-type siblings (J) and rtf1^kt641 mutants (K). Expression of her1 is reduced at the transcriptional level in rtf1^kt641 mutants. Embryos are at the 10- or 12-somite stage and the anterior is top in (A–K). (L) Reverse transcription–PCR analysis of transcript abundance for genes involved in somitogenesis. Data are presented as means+s.d. from triplicate assays.