Figure 2.

Construction of plasmids pCC-s05 and pRO-ilp (A), and physical maps of their killing and regulatory elements (B). stv or s, Streptavidin gene; T7 RNA pol or pol, T7 RNA polymerase gene (T7 gene 1); lys, T7 lysozyme gene (T7 gene 3.5); kan, kanamycin-resistance gene; luc^ΔC, a truncated luc gene encoding an N-terminal fragment of luciferase; MCS, a fragment of pUC18 containing part of the multiple cloning site. B, BamHI; Bg, BglI; D, DraI; Ea, EagI; EI, EcoRI; EV, EcoRV; H, HindIII; Hp, HpaI; K, KpnI; N, NheI; Nr, NruI; P, PstI; Sa, ScaI; Sc, SacI; Sl, SalI; Sm, SmaI. Unique cleavage sites are marked with asterisks.