Figure 3.

Kinetics of the induced suicide of P. putida cultures. Bacteria were grown to a mid-exponential growth phase in LB medium containing 3MB, ampicillin, and kanamycin. After washing with LB, cells were diluted 100-fold with LB supplemented with antibiotics with or without 3MB (A), or resuspended in the same medium without 3MB (B), and further incubated. Broken lines with open symbols refer to the additional presence of IPTG in LB. Plasmid combinations: s04, pCC-s04/pRO-ilp; s05, pCC-s05/pRO-ilp. CFU, colony forming unit. Each data point is the mean of two or three independent experiments.