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. 2007 Aug 1;38(4):371–377. doi: 10.1111/j.1365-2052.2007.01632.x

Table 4.

Genetic marker panel for cat parentage and identification.

Power of exclusion (PE) (min–max)
Marker Cat Chr. Nucleotide repeat Label Final primer concentration (μM)5 Breeds Random-bred
FCA069 B4 AC VIC 0.20 0.1324–0.5336 0.3958–0.5948
FCA075 E2 TG NED 0.10 0.1442–0.5771 0.4240–0.5992
FCA105 A2 TG PET 0.20 0.2221–0.5585 0.6110–0.7101
FCA1491 B1 TG PET 0.18 0.1783–0.5995 0.3586–0.5767
FCA220 F2 CA FAM 0.30 0.0000–0.3383 0.1851–0.4221
FCA229 A1 GT NED 0.25 0.0452–0.5131 0.3927–0.5813
FCA3101 C2 (CA)5TA(CA)7TA(CA)8 FAM 0.30 0.1196–0.5256 0.3417–0.5611
FCA4412 D3 TAGA VIC 0.15 0.2061–0.5774 0.3388–0.5505
FCA6784 A1 AC NED 0.25 0.0415–0.4908 0.3016–0.5715
AMEL3 XY — N/A N/A
ZFXY3 XY — PET 0.20 N/A N/A
Total PE 0.9008–0.9979 0.9947–0.9987
1

Markers that are of the first 10 published feline microsatellites (Menotti-Raymond & O'Brien 1995).

2

A marker that is currently included in the feline forensic panel (Menotti-Raymond et al. 2005).

3

The two markers on the X and Y chromosomes were added to the panel after the comparison test (Pilgrim et al. 2005).

4

Newly designed primers presented herein for FCA678 generate a product 30 bp less than originally published primers.

5

Forward and reverse primers (Table 2) are used in equal concentrations to make combined concentrations for each marker. Final PCR reaction volumes were 15 μl. The suggested PCR conditions include a 5-min denaturation at 95 °C, followed by 35 cycles of denaturation at 95 °C for 1 min, annealing at 58 °C for 30 s and extension at 72 °C for 30 s, with a final 30-min extension at 72 °C.