Table 5.

Suggested conversion of letter nomenclature to allele size for the cat parentage and identification panel.

	Letter nomenclature for alleles
Marker	C	D	E	F	G	H	I	J	K	L	M	N	O	P	Q	R	S	T	U	V	W	X	377
FCA069					93+4	95	97	99	101	103	105 (21)	107+4	109	111	113	115	117						+5
FCA075	104	106	108	110	112	114	116	118	120	122	124 (23)	126	128	130	132+7	134	136	138	140	142	144	146	+4
FCA105					173	175	177	179	181	183	185 (16)	187	189	191	193	195	197−5	199	201	203	205	207	−2
FCA149					122+1	124	126	128+1	130	132	134 (20)	136	138	140	142								+2
FCA229					150	152	154	156	158	160	162 (21)	164	166	168−2	170	172	174	176					0
FCA310					112	114	116	118	120	122	124 (15)	126	128	130	132	134	136	138−2	140				−2
FCA441				145	147	149	151	153	155+0	157	159 (12)+0	161	163	165	167	169	171	173					+2
FCA678						186	188	190	192	194−2	196 (17)	198	200	202	204	206							0
FCA220							208	210	2121	2141	216 (18)	218	220	222	224	226	228						+2

¹A 1-bp insertion has been noted in marker FCA220, producing additional alleles L1 (215 bp) and K1 (213 bp).

All allele sizes were determined on an ABI 3730 DNA Analyzer (Applied Biosystems). Primers for marker FCA678 have been redesigned from the original publication (Menotti-Raymond et al. 1999), producing a shorter product. Alleles that are underlined have been sequenced for homozygous individuals from different breeds or populations. The actual nucleotide length can be determined by the addition or subtraction of the noted number of base pairs that is presented alongside the allele. The numbers of repeats in the core unit of the microsatellite are presented in parentheses for the middle (M) allele. The number of repeats was directly determined for the alleles that were sequenced and interpolated for the M allele. The anticipated conversion required for the ABI 377 is presented in the last column as the number of base pairs that need to be added or subtracted when compared with the ABI 3730.