Fig. 1.

Drug-resistant mutants – the AS lineage. Mutants of P. chabaudi cloned isolate AS have been selected by passage in the presence of pyrimethamine, low, intermediate and high chloroquine concentrations, artesunate and artemisinin, to give lines and clones AS-Pyr (Walliker et al., 1975), AS-3CQ (Rosario, 1976; Carlton et al., 1998), AS-15CQ and AS-30CQ (Padua, 1981), AS-ATN and AS-ART (Afonso et al., 2006) respectively. An asterisk denotes that these clones have been used to generate genetic crosses with the cloned isolate, AJ. The genetic cross investigated in this study used AS-ART and AJ as parental clones. The mutations shown, V739F and V770F, refer to those in UBP-1, described in this study.