Table 5.
Adenine deaminase activity of B. burgdorferi clones.
| Clone | Adenine deaminase activitya (pmol min−1 mg−1 total cell lysate ± SD) |
|---|---|
| A3-M9 | 17 ± 2.9 |
| A3-M9 lp36-minus | −0.4 ± 0.7b |
| A3-M9 lp36-minus/lp36-gent | 19 ± 0.2 |
| A3-M9 lp36-minus/pBSV2G | −0.1 ± 0.6b |
| A3-M9 lp36-minus/pBSV2G bbk17 | 8.5 ± 0.2 |
| A3-M9 Δbbk17::flgBp-kan/pBSV2G | −0.2 ± 0.5b |
| A3-M9 Δbbk17::flgBp-kan/pBSV2G bbk17 | 15 ± 2.4 |
a
Adenine deaminase activity was determined by the rate of hypoxanthine produced over a 30 min period and expressed as the average change in hypoxanthine per minute per milligram of total protein in the cell lysate. Data represent the average of three experiments. Error is show as the standard deviation (SD) from the mean.
b
No adenine deaminase activity was detected. A negative rate of hypoxanthine production was observed as a result of consumption of background levels of hypoxanthine by unidentified enzymatic activities present in the cell lysates.