Table 2.
Oligonucleotide primers
| Gene | Primer name | Primer sequences (5′–3′) | Application |
|---|---|---|---|
| fimR | rfimR-F | ATGATTAGTATCGTACTC | The full-length fimR ORF amplification |
| rfimR-R | CTATTGCCAATCCACTAA | ||
| fimRF | TAGGCTTTTGCCAGATTGGA | Construction of fimR mutation | |
| fimRR | CCAAATCGGGAATTTAGCTC | ||
| ermF-ermAM | ErmF | CACCGTCATATGCGATAGCTTCCGCTATTGCT | ermF-ermAM cassette amplification |
| ErmR | GGAACTCATATGTCCCCGAAGCTGTCAGTAGT | ||
| fimA | fimAF | CGGAACGAATAACCCAGAGA | Real-time PCR |
| fimAR | CTGACCAACGAGAACCCACT | ||
| fimAProm-F | CGACGCTATATGCAAGACAA | The biotin-labeled promoter region | |
| fimAProm-R | Bio-TGTAACGGGTTCTGCCTCGT | ||
| Mfa1 | MfaProm-F | CTCTCGCGAGGGTCAATATC | The biotin-labeled promoter region |
| MfaProm-R | Bio-CGTCTTACCGGCTTCCCTAT | ||
| 67KD121F | CAGATGGGTTGTTGCTCA | The biotin-labeled coding region | |
| 67KD121R | Bio-ATAGAAAGTGCTGCTGGTAG | ||
| MfaF | CAGATGGGTTGTTGCTCA | Real-time PCR | |
| MfaR | GAAAGTGCTGCTGGTAG | ||
| MfaTSR1 | CTCGTTATCACATATCCGAACC | Identification of transcriptional start site | |
| MfaTSR2 | GAAGCAAAGCCCAATGAGAG | ||
| MfaTSR3 | CCGCTCGACTCACGAGACTA | ||
| MfaTSR4 | CACGACATAGAGTGTTCAGA | ||
| MfaTSR5 | CGTCTTGCCGACAGCAGAAT | ||
| MfaTSF1 | AGCCGGTAAGACGTAGCTGA | ||
| MfaTSF2 | ACGTAGAAGACAGCAGAATA | ||
| MfaTSF3 | TCTCTCGCGAGGGTCAATA | ||
| 16S | 16sRNA-F | TGGGTTTAAAGGGTGCGTAG | Real-time PCR |
| 16sRNA-R | CAATCGGAGTTCCTCGTGAT | ||
| rgpA | rgpAF | CAACCAGTCTTGGGCTTCTC | Real-time PCR |
| rgpAR | CCACCATAGCAAACATACCG |