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. Author manuscript; available in PMC: 2007 Sep 10.
Published in final edited form as: Mol Cell Endocrinol. 2007 Apr 6;271(1-2):55–63. doi: 10.1016/j.mce.2007.04.002

Figure 4. Dlk1 Regulates Pit-1 Transcriptional Activation in MCF7 cells.

Figure 4

-130GHp-Luc was transfected into MCF-7 cells along with Dlk1 or Pit-1 alone, or with Dlk1 and Pit-1 together to determine if Dlk1 can repress Pit-1 activated transcription. As a control, the 4X antioxidant response element reporter (4XARE-Luc) was transfected into MCF-7 cells along with Dlk1 or Nrf2 alone, or with Dlk1 and Nrf2 together to determine if Dlk1 can repress transcription mediated by another unrelated transcription factor. Empty PCI expression vector was used to ensure that each well was transfected with equal amounts of DNA. Values are the mean ± standard deviation of three 3 separate experiments with duplicate samples per experiment. *: P<0.05.