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. 2007 Sep 13;117(10):2974–2982. doi: 10.1172/JCI31344

Figure 4. Hypercholesterolemia results in increased PD-L1, PD-L2, and B7-1 expression by APCs.

Figure 4

LDLR–/– mice were fed control diet (con) or cholesterol-containing diet for 3 or 10 weeks before sacrifice and tissue harvesting. Iliac lymph node cell suspensions (A) and peritoneal macrophages (B) were stained with fluorescent antibodies specific for CD11c and F4/80, respectively, and both populations were also stained with fluorescent antibodies specific for PD-L1, PD-L2, and B7-1. Values represent the percentage of the CD11c+ or F4/80+ cells that stained positive for the indicated antibodies. *P < 0.05, †P < 0.01 versus control diet. n = 4 per group. Data are from 1 of 2 experiments with similar results. (C) ORO staining for lipid in peritoneal macrophages isolated from mice on cholesterol or control diets. Original magnification, ×100.