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. Author manuscript; available in PMC: 2008 Jul 27.
Published in final edited form as: J Mol Biol. 2007 May 8;370(5):856–869. doi: 10.1016/j.jmb.2007.04.076

Figure 5.

Figure 5

RNase T1 probing on the TSV IRES showing the cleavage pattern that resulted when the IRES was allowed to fold in the presence of MgCl2. Filled circles indicate that the G base was strongly protected from the RNase upon the addition of the cation. Open circles represent G bases that were weakly protected. Open stars are indicative of G bases that were weakly cleaved more in the presence of the cation. Filled stars depict G bases that were strongly cleaved in the presence of the Mg2+ cation.