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Activation of Acanthamoeba myosin IC by phosphorylation of its heavy chain by PAK1
| Kinase | Mg2+-ATPase activity, s−1
|
|
|---|---|---|
| − actin | + actin | |
| None | 0.5 | 1.3 |
| PAK1 | 0.5 | 7.5 |
| 35K | 0.5 | 8.5 |
Myosin IC was incubated in the presence of ATP either alone or with PAK1 or 35K for 30 min at 30°C. The Mg2+-ATPase activity of the myosin was then measured in the absence or presence of 90 μM F-actin, as described in more detail in text.
