Figure 1.

Separation of polypeptides copurifying with human RNase P activity eluted from the S-12 column (A) in a 12% polyacrylamide gel that contains 0.1% SDS (B). The lane numbers indicate the fractions (0.5 ml each) from the column. (A) Assay for RNase P activity across the column. Lane C, no added column fraction. Lane O, load sample added. Other lanes as indicated above. S, substrate; P, tRNA-containing product. The 5′ leader portion of the product runs near the bottom of the gel. (B) The arrowheads indicate the positions of proteins discussed in the text. The positions of molecular weight markers are shown to the left.