Figure 2.

Immunoprecipitation of RNase P activity in the second glycerol gradient fractions that contained the peak of activity with sera from SSc patients labeled as ThFi (A), ThLo (B), and ThMi (C). RNase P activity was assayed as indicated for either 10 or 30 min. (A) Lane C, no added antiserum or enzyme. Lanes 1, RNase P added; no antiserum. Lanes 2, protein A-Sepharose beads with no added antiserum; RNase P added. Lanes 3 and 4, beads coated with a different rabbit anti-C5 protein (E. coli) in each case and RNase P added. Lanes 5, beads coated with ThFi serum and RNase P added. (B) Same as A, except lanes 3 contain beads coated with rabbit preimmune serum and lanes 5 contain beads coated with ThLo serum. (C) Same as B, except lanes 5 contain beads coated with ThMi serum.