Figure 4.

cDNA sequence and translated polypeptide sequence for RPP30 (Left) and RPP38 (Right). The nucleotide sequence is numbered from the start of the total sequence determined. The amino acid sequence is numbered from the first methionine residue and is shown in boldface letters. The portions of the peptide sequences that correspond to tryptic peptide fragments derived from authentic Rpp30 and Rpp38 are underlined. Note that for RPP30, the cDNA sequence has one base substitution difference from the predicted sequence and the cDNA sequence for RPP38 has two base substitution differences from the predicted sequence. These differences, which might well be expected from cDNA clones derived from human tissue different from HeLa cells, give rise to an I → S change in the peptide sequence at position 189 in Rpp30 and to an A → S change in the peptide sequence at position 24 and an A → V change at position 114 in Rpp38.