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. 1997 Feb 18;94(4):1107–1112. doi: 10.1073/pnas.94.4.1107

Figure 2.

Figure 2

Chaperonin-mediated protein folding in the presence of macromolecular crowding agents. (A) Chaperonin-dependent refolding of rhodanese in buffer (▪) and 30% Ficoll 70 (○, •) with GroEL and ATP in the presence (○) or absence (•) of GroES. The arrow in reaction ○ marks the addition of 15 mM CDTA after 7.5 min to stop chaperonin-mediated folding. Incubation continued for the times indicated at which enzyme activities were determined. (B) GroEL/ES-dependent refolding of MDH in buffer (•) and Ficoll 70 solution (○). (C) Refolding of rhodanese (•, ○) and MDH (▪, □) in Ficoll 70 in the presence of GA-GroEL (○, □) or GroEL337/349 (•, ▪). Preformed rhodanese-GroEL and MDH–GroEL complexes (0.25 μM) were reactivated in 30% Ficoll 70 solutions in the presence of increasing concentrations of GA-GroEL or GroEL337/349, essentially as described for A.