Figure 5.

Protein folding in concentrated Xenopus egg extracts. (A) Kinetics of chaperonin-mediated refolding of rhodanese in buffer and in a concentrated cytosol extract (≈200 mg of protein per ml). Experiments were performed in the presence and absence of GroES. The maximal refolding yield in buffer is set to 100%. (B) Effects of trap-GroEL on chaperonin-assisted rhodanese refolding in buffer and in cytosol extracts (original concentration of ≈200 mg/ml and 1:2 diluted). An 8-fold molar excess of trap-GroEL over wild-type GroEL was used in the experiments. Refolding reaction cycles proceeded for 15 min. The yield of rhodanese refolding in buffer in the absence of trap-GroEL is set to 100%.