. 1997 Feb 18;94(4):1124–1129. doi: 10.1073/pnas.94.4.1124

Table 2.

List of mutants generated by cassette mutagenesis of wild-type synthetic pfDHFR domain

Construct	Field isolates with equivalent mutation^*	Drug susceptibility^†		Amino acid residue of DHFR domain^‡
Construct	Field isolates with equivalent mutation^*	Pyr	Cyc	16	51	59	108	164
Wild type	3D7, SL/D6	S	S	Ala	Asn	Cys	Ser	Ile
A16V^§				Val	Asn	Cys	Ser	Ile
N51I^§				Ala	Ile	Cys	Ser	Ile
C59R^§				Ala	Asn	Arg	Ser	Ile
S108N	HB3	MR	LR	Ala	Asn	Cys	Asn	Ile
S108T^§				Ala	Asn	Cys	Thr	Ile
I164L^§				Ala	Asn	Cys	Ser	Leu
A16V + S108T	FAC8, FAC3, UPA, FCB, It.G2.F6	S	HR	Val	Asn	Cys	Thr	Ile
A16V + S108N^§				Val	Asn	Cys	Asn	Ile
N51I + S108N	7G8, It.D12	MR	MR	Ala	Ile	Cys	Asn	Ile
C59R + S108N	K1, V1	MR	MR	Ala	Asn	Arg	Asn	Ile
N51I + C59R + S108N	W2	HR	MR	Ala	Ile	Arg	Asn	Ile
C59R + S108N + I164L	Cs1-2	HR	HR	Ala	Asn	Arg	Asn	Leu
N51I + C59R + S108N + I164L	V1/S	HR	HR	Ala	Ile	Arg	Asn	Leu

Point mutations of the DHFR were previously described (9, 11).

^†

Based on data previously reported (9, 11); S, sensitive; LR, low resistance; MR, moderate resistance; HR, high resistance.

^‡

Boldface indicates mutated residue(s).

^§

Not found in nature.