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. 1997 Feb 18;94(4):1130–1135. doi: 10.1073/pnas.94.4.1130

Figure 3.

Figure 3

Effect of antiserum on PTTH activity in the in vitro assay. (A) Dose-dependent effect of the purified 45-kDa PTTH on ecdysone secretion by the ring glands in vitro. Each datum point represents the mean (±SEM) of four separate determinations. (B) Inhibition of the activity of purified PTTH by the anti-PTTH antiserum. Various doses of anti-PTTH anti-serum and purified PTTH (0.5 μg) were incubated together and the mixture was then assayed using the ring gland in vitro assay as in A.