Figure 2.

Stability of α/β complexes in various detergents. Oocytes injected with the indicated cRNAs (Tα1, 20 ng of cRNA per cell; Rα1, Rα2, and Rα3, 40 ng of cRNA per cell; Mβ2, 4 ng of cRNA per cell) were [³⁵S]methionine-labeled for 3 h and chased for 20 h. Extracts of subsets of these cells were prepared using 0.5% Triton X-100 (lanes 1–5) or 1% digitonin (lanes 6–10) for extraction and mAb 426 for immunoprecipitation. Immunoprecipitates were analyzed by SDS/PAGE and fluorography. Positions of relative molecular mass markers (in kDa) are indicated on the left. Abbreviations are as in Fig. 1.