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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: Cell Calcium. 2007 Jan 24;42(3):261–270. doi: 10.1016/j.ceca.2006.12.002

Figure 2. Validation of anti-pS934.

Figure 2

(A) HEK cells transfected with empty vector (lanes 1-2), or cDNA encoding wild-type IP3R3 (IP3R3SSS; lanes 3-4), IP3R3 mutated at S916, S934, S916, and S1832 (IP3R3AAA; lanes 5-6), mutated at S934 (IP3R3SAS; lanes 7-8), mutated at S916 and S1832 (IP3R3ASA; lanes 9-10) or wild-type IP3R1 (IP3R1SS; lanes 11-12) were incubated with or without 10 μM forskolin for 5 min as indicated, and lysates were prepared and probed with anti-pS934 (upper panel), TL3 (lower panel; lanes 1-10), or CT1 (lower panel; lanes 11-12). (B) COS cells transfected with cDNA encoding IP3R3SSS were incubated without (upper panels) or with (lower panels) 10 μM forskolin for 10 min, fixed, and stained with DAPI to visualize nuclei (panels iii and iv), TL3 to visualize IP3R3 (panels v and vi), and anti-pS934 to visualize IP3R3 phosphorylated at S934 (panels vii and viii).