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Western blot analysis of eNOS, iNOS and total nitrotyrosine (NO2-T) in LV: The tissue homogenates from Sham, aortic-stenosis (AS), Sham + CZ and AS+CZ mice were prepared. The levels of eNOS, iNOS and protein nitrotyrosine were measured by immuno-blots using respective antibodies. β-actin was used for equal loading; graphs represent scan data for band intensities; *p<0.05 compared to sham; ** p<0.05 compared to AS; n=6 in each group.
