(A-C) Scanning electron microscope images of GMR>Par-1(KN) (A), GMR>Par-1 (B) and GMR>Par-1; mts/+ (C). (D) Localization of Arm (A, red) and Dpatj (A’, green) in a kinase-inactive mutant form of Par-1 (Par-1(KN)) overexpressed by GMR-Gal4. (E) Par-1 overexpression by GMR-Gal4 causes severe mislocalization of Arm (E, red) and Dpatj (E’, green), resulting in cell polarity defects (E”, arrows). The same images shown in Fig. 3B are provided here for phenotype comparison purposes. (F) Par-1 overexpression by GMR-Gal4 in the mts/+ heterozygous background (GMR>Par-1; mts/+) shows further reduction or loss of Arm (F, red) and Dpatj (F’, green), compared to GMR>Par-1 (E).