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. Author manuscript; available in PMC: 2008 Jan 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2007 Jan;48(1):18–26. doi: 10.1167/iovs.06-0619

FIGURE 2.

FIGURE 2

Characterization of caveolae/lipid raft protein components in hRVE cells. Membrane lipids were fractionated to enrich for caveolae/lipid rafts and nonraft components. Each membrane fraction was separated on SDS-PAGE. Western blot analysis for the caveolae marker caveolin-1, the lipid raft marker flotillin-1, the plasma membrane marker Na+/K+ATPase, PKC-α, and Erk1/2 was performed to analyze protein distribution in each fraction. Fractions designated caveolae/raft and nonraft membrane are indicated.